-
Purpose(Empty Backbone) Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneLentiviral
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Resource Information
-
Supplemental Documents
-
Addgene Notes
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor states that discrepancies found by Addgene's NGS should not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
BFP dest clone was a gift from Jacob Corn (Addgene plasmid # 71825 ; http://n2t.net/addgene:71825 ; RRID:Addgene_71825) -
For your References section:
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE. Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481. 10.1038/nbt.3481 PubMed 26789497
