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Addgene

pCR1054
(Plasmid #71822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SPynCas9
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR1054 was a gift from Jacob Corn (Addgene plasmid # 71822 ; http://n2t.net/addgene:71822 ; RRID:Addgene_71822)

  • For your References section:

    Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE. Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481. 10.1038/nbt.3481 PubMed 26789497
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