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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
(Plasmid #71811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTattB
  • Backbone manufacturer
    (GenBank EF362409.1)
  • Backbone size w/o insert (bp) 8052
  • Total vector size (bp) 12493
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
  • Insert Size (bp)
    4441

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Brian Sauer - pBS302 (Addgene plasmid# 11925)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM for expression of FLP-inducible Dam-LAM protein was made by cloning the Drosophila Lamin Dm0 open reading frame from the previously described pDamMyc-Dm0 plasmid (Pickersgill et al., 2006, PMID=16878134) between the AscI and NotI sites in p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM was a gift from Bas van Steensel (Addgene plasmid # 71811 ; http://n2t.net/addgene:71811 ; RRID:Addgene_71811)

  • For your References section:

    Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila. Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van Steensel B. Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 10.1093/nar/gkw176. Epub 2016 Mar 21. 10.1093/nar/gkw176 PubMed 27001518
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