p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]
(Plasmid #71809)

• Purpose: Generating transgenic flies for FLP-inducible DamID
• Depositing Lab: Bas van Steensel
• Publication: Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 10.1093/nar/gkw176. Epub 2016 Mar 21.

Backbone

• Vector backbone: pUASTattB
• Backbone manufacturer: (GenBank EF362409.1)
• Backbone size w/o insert (bp): 8052
• Total vector size (bp): 10645
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
• Insert Size (bp): 2593

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Brian Sauer - pBS302 (Addgene plasmid# 11925)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] plasmid vector was constructed from the following elements: Drosophila minimal hsp70 promoter (“min.hsp70P”), STOP#1 cassette flanked by two unidirectional FRT sites, open reading frame encoding Dam-Myc fusion protein, polylinker consisting of AscI, SwaI, FseI, AsiSI and NotI sites, SV40 polyadenylation signal, attB site for phiC31 integrase-mediated transgenesis, pUC backbone, Drosophila mini-white marker and loxP site. The elements were assembled in the indicated order; the last five elements were taken as a whole from pUASTattB vector (Bischof et al., 2007, PMID=17360644; GenBank EF362409.1). The p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] vector is designed for cloning and expression of FLP-inducible N-terminal Dam fusion proteins.