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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC
(Plasmid #71808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTattB
  • Backbone manufacturer
    (GenBank EF362409.1)
  • Backbone size w/o insert (bp) 8052
  • Total vector size (bp) 11824
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC
  • Insert Size (bp)
    3772

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC for expression of Dam[4-HT-intein@L127C]-PC protein was made by cloning the Drosophila Polycomb open reading frame from the previously described pNDamMyc-Pc plasmid (Tolhuis et al., 2006, PMID=16628213) between the AscI and NotI sites in p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC was a gift from Bas van Steensel (Addgene plasmid # 71808 ; http://n2t.net/addgene:71808 ; RRID:Addgene_71808)

  • For your References section:

    Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila. Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van Steensel B. Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 10.1093/nar/gkw176. Epub 2016 Mar 21. 10.1093/nar/gkw176 PubMed 27001518
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