p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
(Plasmid #71805)

• Purpose: Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID
• Depositing Lab: Bas van Steensel
• Publication: Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 10.1093/nar/gkw176. Epub 2016 Mar 21.

Backbone

• Vector backbone: pUASTattB
• Backbone manufacturer: GenBank EF362409.1
• Backbone size w/o insert (bp): 8052
• Total vector size (bp): 10672
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
• Insert Size (bp): 2620

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] plasmid vector was constructed from the following elements: Drosophila full-length hsp70 promoter (“FL.hsp70P”), Dam[4-HT-intein@L127C]-Myc cassette (in-frame fusion of E.coli DNA adenine methyltransferase (containing 4-hydroxytamoxifen-inducible intein [Buskirk et al., 2004, PMID=15247421] inserted immediately before L127C mutation) and Myc epitope tag), polylinker consisting of AscI, SwaI, FseI, AsiSI and NotI sites, SV40 polyadenylation signal, attB site for phiC31 integrase-mediated transgenesis, pUC backbone, Drosophila mini-white marker and loxP site. The elements were assembled in the indicated order; the last five elements were taken as a whole from pUASTattB vector [Bischof et al., 2007, PMID=17360644; GenBank EF362409.1]. The p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open] plasmid vector is designed for cloning and expression of N-terminal Dam[4-HT-intein@L127C] fusion proteins