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Addgene

pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
(Plasmid #71761)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4941
  • Total vector size (bp) 7404
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mGFP
  • Insert Size (bp)
    777
  • Tag / Fusion Protein
    • Palmitoylation signal (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Synaptophysin
  • Insert Size (bp)
    951
  • Tag / Fusion Protein
    • mRuby (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby was a gift from Liqun Luo (Addgene plasmid # 71761 ; http://n2t.net/addgene:71761 ; RRID:Addgene_71761)

  • For your References section:

    Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic Input-Output Mapping. Beier KT, Steinberg EE, DeLoach KE, Xie S, Miyamichi K, Schwarz L, Gao XJ, Kremer EJ, Malenka RC, Luo L. Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015. 10.1016/j.cell.2015.07.015 PubMed 26232228
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