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His-TEV-Aurein1.2_(+36GFP)-LPETG-His
(Plasmid #71755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5181
  • Total vector size (bp) 6027
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, transform into BL21 Star (DE3) cells. Induce at 1.0 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aurein1.2_(pos36_GFP)-LPETG-His
  • Species
    Synthetic
  • Insert Size (bp)
    882
  • GenBank ID
  • Tags / Fusion Proteins
    • 6XHis (C terminal on insert)
    • Sortase (LPETG) (C terminal on insert)
    • 6XHis (N terminal on insert)
    • TEV Protease (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site xhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-TEV-Aurein1.2_(+36GFP)-LPETG-His was a gift from David Liu (Addgene plasmid # 71755 ; http://n2t.net/addgene:71755 ; RRID:Addgene_71755)

  • For your References section:

    Discovery and Characterization of a Peptide That Enhances Endosomal Escape of Delivered Proteins in Vitro and in Vivo. Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, Schepartz A, Chen ZY, Liu DR. J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 10.1021/jacs.5b05694. Epub 2015 Oct 30. 10.1021/jacs.5b05694 PubMed 26465072
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