His-TEV-U_(+36GFP)-Cre
(Plasmid
#71750)
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PurposeHis-TEV-U+36GFP-Cre for E.Coli expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5181
- Total vector size (bp) 7167
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor protein expression, transform into BL21 Star (DE3) cells. Induce at 1.0 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU_(pos36GFP)-Cre
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SpeciesSynthetic
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Insert Size (bp)1986
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Tags
/ Fusion Proteins
- TEV protease (N terminal on insert)
- 6X His (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-TEV-U_(+36GFP)-Cre was a gift from David Liu (Addgene plasmid # 71750 ; http://n2t.net/addgene:71750 ; RRID:Addgene_71750) -
For your References section:
Discovery and Characterization of a Peptide That Enhances Endosomal Escape of Delivered Proteins in Vitro and in Vivo. Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, Schepartz A, Chen ZY, Liu DR. J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 10.1021/jacs.5b05694. Epub 2015 Oct 30. 10.1021/jacs.5b05694 PubMed 26465072
