Aurein1.2_(+36GFP)-Cre
(Plasmid
#71748)
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PurposeAurein1.2+36GFP-Cre fusion expression in E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5181
- Total vector size (bp) 7122
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor protein expression, transform into BL21 Star (DE3) cells. Induce at 1.0 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name(GGS)9_Aurein1.2+36GFP-Cre
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SpeciesSynthetic
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Insert Size (bp)1941
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Tag
/ Fusion Protein
- 6XHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Aurein1.2_(+36GFP)-Cre was a gift from David Liu (Addgene plasmid # 71748 ; http://n2t.net/addgene:71748 ; RRID:Addgene_71748) -
For your References section:
Discovery and Characterization of a Peptide That Enhances Endosomal Escape of Delivered Proteins in Vitro and in Vivo. Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, Schepartz A, Chen ZY, Liu DR. J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 10.1021/jacs.5b05694. Epub 2015 Oct 30. 10.1021/jacs.5b05694 PubMed 26465072