pTA7001-DEST
(Plasmid #71745)

• Purpose: (Empty Backbone) GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible
• Depositing Lab: Roger Innes
• Publication: Gu et al Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. Epub 2011 Feb 22.

Backbone

• Vector backbone: pBI101
• Backbone manufacturer: Clontech
• Backbone size (bp): 9000
• Modifications to backbone: T-DNA region of pBI101 was replaced with a new T-DNA sequence that enables dexamethasone-inducible gene expression of a gene of interest. Construction of pTA7001 is described in Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11, 605–612. pTA7001 was further modified to contain a GATEWAY destination cassette inserted between Xho1 and Spe1 sites.
• Vector type: Plant Expression
• Promoter: GAL4
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jean Greenberg at the University of Chicago.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612