UCP3 promoter EP1
(Plasmid #71743)

• Purpose: Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity
• Depositing Lab: Heinrich Taegtmeyer
• Publication: Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.673988.

Backbone

• Vector backbone: pGL4.12[luc2CP]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4421
• Total vector size (bp): 11467
• Modifications to backbone: None
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Uncoupling Protein 3 promoter
• Alt name: UCP3 promoter
• Alt name: Slc25a9 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 7042
• Mutation: Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2
• Entrez Gene: Ucp3 (a.k.a. AI645527, Slc25a9, UCP-3)
• Promoter: UCP3
• Tag / Fusion Protein:
  • Luciferase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: RV3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry