pBAD-TRP32
(Plasmid
#71730)
-
Purposeinducible expression of TRP32 in E.coli
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBAD/Thio-TOPO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4454
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTRP32
-
Alt nameVLPT
-
SpeciesEhrlichia chaffeensis
-
Insert Size (bp)594
-
GenBank IDAF121232
- Promoter pBAD
-
Tags
/ Fusion Proteins
- Thioredoxin (N terminal on backbone)
- 6xHIS (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer pTrcHis-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-TRP32 was a gift from Jere McBride (Addgene plasmid # 71730 ; http://n2t.net/addgene:71730 ; RRID:Addgene_71730) -
For your References section:
A variable-length PCR target protein of Ehrlichia chaffeensis contains major species-specific antibody epitopes in acidic serine-rich tandem repeats. Luo T, Zhang X, Wakeel A, Popov VL, McBride JW. Infect Immun. 2008 Apr;76(4):1572-80. doi: 10.1128/IAI.01466-07. Epub 2008 Jan 22. 10.1128/IAI.01466-07 PubMed 18212082