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Addgene

pBAD-TRP32
(Plasmid #71730)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/Thio-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4454
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRP32
  • Alt name
    VLPT
  • Species
    Ehrlichia chaffeensis
  • Insert Size (bp)
    594
  • GenBank ID
    AF121232
  • Promoter pBAD
  • Tags / Fusion Proteins
    • Thioredoxin (N terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-TRP32 was a gift from Jere McBride (Addgene plasmid # 71730 ; http://n2t.net/addgene:71730 ; RRID:Addgene_71730)

  • For your References section:

    A variable-length PCR target protein of Ehrlichia chaffeensis contains major species-specific antibody epitopes in acidic serine-rich tandem repeats. Luo T, Zhang X, Wakeel A, Popov VL, McBride JW. Infect Immun. 2008 Apr;76(4):1572-80. doi: 10.1128/IAI.01466-07. Epub 2008 Jan 22. 10.1128/IAI.01466-07 PubMed 18212082
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