Gal4-VP16
(Plasmid #71728)

• Purpose: plasmid expressing fusion construct of Gal4 DBD and VP16 AD
• Depositing Lab: Lea Sistonen
• Publication: Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11.

Backbone

• Vector backbone: pECE
• Backbone size w/o insert (bp): 2924
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal4 DBD(1-147)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 441
• GenBank ID: NM_001184062.1
• Entrez Gene: GAL4 (a.k.a. YPL248C, GAL81)
• Tag / Fusion Protein:
  • VP16 AD (413-490) (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Steven Triezenberg
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry