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Addgene

Gal4-VP16-HSF1 WT
(Plasmid #71726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE
  • Backbone size w/o insert (bp) 2924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4 DBD HSF1 VP16 AD
  • Alt name
    fusion protein consisting of Gal4 DBD HSF1 Regulatory Domain and VP16 activator domain
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast); Herpes Simplex Virus protein VP16
  • GenBank ID
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4-VP16-HSF1 WT was a gift from Lea Sistonen (Addgene plasmid # 71726 ; http://n2t.net/addgene:71726 ; RRID:Addgene_71726)

  • For your References section:

    Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Budzynski MA, Puustinen MC, Joutsen J, Sistonen L. Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11. 10.1128/MCB.00816-14 PubMed 25963659
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