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Addgene

pcDNA-HSF1Δ∼PRD
(Plasmid #71725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1/Myc-His (-) A
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphorylation-deficient HSF1
  • Alt name
    HSF1Δ∼PRD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    15 phosphorylation sites in RD mutated to Alanine
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Tag / Fusion Protein
    • mac 6xHIS (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HSF1Δ∼PRD was a gift from Lea Sistonen (Addgene plasmid # 71725 ; http://n2t.net/addgene:71725 ; RRID:Addgene_71725)

  • For your References section:

    Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Budzynski MA, Puustinen MC, Joutsen J, Sistonen L. Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11. 10.1128/MCB.00816-14 PubMed 25963659
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