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Addgene

pcDNA-hHSF1 WT
(Plasmid #71724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1/Myc-His (-) A
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSF1
  • Alt name
    heat shock transcription factor 1
  • Alt name
    HSTF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • GenBank ID
    NM_005526.2
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Tag / Fusion Protein
    • MYC 6xHIS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-hHSF1 WT was a gift from Lea Sistonen (Addgene plasmid # 71724 ; http://n2t.net/addgene:71724 ; RRID:Addgene_71724)

  • For your References section:

    Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Budzynski MA, Puustinen MC, Joutsen J, Sistonen L. Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11. 10.1128/MCB.00816-14 PubMed 25963659
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