pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
(Plasmid #71719)

• Purpose: Expressing a GFP-tagged degron in the soma of C. elegans
• Depositing Lab: Abby Dernburg
• Publication: Zhang et al Development. 2015 Nov 9. pii: dev.129635.

Backbone

• Vector backbone: pCFJ151
• Backbone size w/o insert (bp): 7373
• Total vector size (bp): 9593
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: auxin-responsive protein IAA17
• Alt name: IAA17
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 135
• Mutation: minimal degron sequence (71-114aa) with start codon
• Entrez Gene: AXR3 (a.k.a. AT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17)
• Promoter: eef-1A.1 (eft-3)
• Tag / Fusion Protein:
  • EmGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGCCTAAAGATCCAGCCAAACCTCC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3