pET28a-MSP1D1deltaH4/2
(Plasmid #71712)

• Purpose: Membrane scaffold protein (MSP), half of helix 4 truncated
• Depositing Lab: Gerhard Wagner
• Publication: Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. Epub 2013 Jan 25.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 5816
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Apolipoprotein A1
• Alt name: MSP
• Species: H. sapiens (human)
• Insert Size (bp): 543
• Entrez Gene: APOA1 (a.k.a. AMYLD3, HPALP2, apo(a))
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • TEV site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promotor
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry