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Addgene

pET28a-MSP1D1deltaH4/2
(Plasmid #71712)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 5816
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Apolipoprotein A1
  • Alt name
    MSP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    543
  • Entrez Gene
    APOA1 (a.k.a. AMYLD3, HPALP2, apo(a))
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • TEV site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promotor
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-MSP1D1deltaH4/2 was a gift from Gerhard Wagner (Addgene plasmid # 71712 ; http://n2t.net/addgene:71712 ; RRID:Addgene_71712)

  • For your References section:

    Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. Hagn F, Etzkorn M, Raschle T, Wagner G. J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. Epub 2013 Jan 25. 10.1021/ja310901f PubMed 23294159
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