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PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1
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Backbone manufacturerInvitrogen (Life Technologies)
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 9830
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha-catenin FRET Sensor
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SpeciesH. sapiens (human)
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Insert Size (bp)4400
- Promoter CMV
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- YPET (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byacat 1-906 from David Rimm (Addgene plasmid # 24194)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
alpha catenin conformation sensor was a gift from Deborah Leckband (Addgene plasmid # 71709 ; http://n2t.net/addgene:71709 ; RRID:Addgene_71709) -
For your References section:
Dynamic visualization of alpha-catenin reveals rapid, reversible conformation switching between tension states. Kim TJ, Zheng S, Sun J, Muhamed I, Wu J, Lei L, Kong X, Leckband DE, Wang Y. Curr Biol. 2015 Jan 19;25(2):218-24. doi: 10.1016/j.cub.2014.11.017. Epub 2014 Dec 24. 10.1016/j.cub.2014.11.017 PubMed 25544608