pJK615
(Plasmid #71699)

• Purpose: Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
• Depositing Lab: T. J. Kappock
• Publication: Additional Acetobacter aceti 1023 thioredoxin-associated genes (unpublished)

Backbone

• Vector backbone: pET32a
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 5701
• Modifications to backbone: excised C-terminal tags
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: thioredoxin
• Alt name: trxA
• Alt name: JW5856
• Alt name: b3781
• Species: Escherichia coli str. K-12 substr. MG1655
• GenBank ID: AAA67582.1
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry