pJK667
(Plasmid
#71690)
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PurposeProduces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6496
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameacetate kinase
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Alt nameackA
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SpeciesEscherichia coli
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GenBank IDNP_416799.1
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Entrez GeneackA (a.k.a. b2296, ECK2290)
- Promoter T7 promoter
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK667 was a gift from T. J. Kappock (Addgene plasmid # 71690 ; http://n2t.net/addgene:71690 ; RRID:Addgene_71690) -
For your References section:
Functional Dissection of the Bipartite Active Site of the Class I Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase. Murphy JR, Mullins EA, Kappock TJ. Front Chem. 2016 May 23;4:23. doi: 10.3389/fchem.2016.00023. eCollection 2016. 10.3389/fchem.2016.00023 PubMed 27242998
