pME-eSIBR
(Plasmid
#71665)
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Purposeintronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2544
- Total vector size (bp) 4051
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Vector typeRNAi ; Multi-site Gateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintronic eSIBR cassette
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Insert Size (bp)1507
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byDavid Turner, University of Michigan
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The original SIBR cassette and instructions for cloning into and chaining SIBR amiRNAs are described in:
Chung, Kwan-Ho, et al. "Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155." Nucleic Acids Research 34.7 (2006): e53-e53.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-eSIBR was a gift from Philip Washbourne (Addgene plasmid # 71665 ; http://n2t.net/addgene:71665 ; RRID:Addgene_71665) -
For your References section:
Improved knockdown from artificial microRNAs in an enhanced miR-155 backbone: a designer's guide to potent multi-target RNAi. Fowler DK, Williams C, Gerritsen AT, Washbourne P. Nucleic Acids Res. 2015 Nov 17. pii: gkv1246. 10.1093/nar/gkv1246 PubMed 26582923