Skip to main content
Addgene

pME-eSIBR
(Plasmid #71665)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71665 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2544
  • Total vector size (bp) 4051
  • Vector type
    RNAi ; Multi-site Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    intronic eSIBR cassette
  • Insert Size (bp)
    1507
  • Promoter None

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Turner, University of Michigan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original SIBR cassette and instructions for cloning into and chaining SIBR amiRNAs are described in:
Chung, Kwan-Ho, et al. "Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155." Nucleic Acids Research 34.7 (2006): e53-e53.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-eSIBR was a gift from Philip Washbourne (Addgene plasmid # 71665 ; http://n2t.net/addgene:71665 ; RRID:Addgene_71665)
  • For your References section:

    Improved knockdown from artificial microRNAs in an enhanced miR-155 backbone: a designer's guide to potent multi-target RNAi. Fowler DK, Williams C, Gerritsen AT, Washbourne P. Nucleic Acids Res. 2015 Nov 17. pii: gkv1246. 10.1093/nar/gkv1246 PubMed 26582923