pME-eSIBR
(Plasmid #71665)

• Purpose: intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)
• Depositing Lab: Philip Washbourne
• Publication: Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv1246.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2544
• Total vector size (bp): 4051
• Vector type: RNAi ; Multi-site Gateway

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: intronic eSIBR cassette
• Insert Size (bp): 1507
• Promoter: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • pME-eSIBR ApE file
• A portion of this plasmid was derived from a plasmid made by: David Turner, University of Michigan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original SIBR cassette and instructions for cloning into and chaining SIBR amiRNAs are described in:
Chung, Kwan-Ho, et al. "Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155." Nucleic Acids Research 34.7 (2006): e53-e53.