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PurposeMammalian expression of hnRNPC
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)/myc-His A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5490
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehnRNPC
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Alt nameheterogeneous nuclear ribonucleoprotein C (C1/C2)
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SpeciesH. sapiens (human)
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Insert Size (bp)879
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Entrez GeneHNRNPC (a.k.a. C1, C2, HNRNP, HNRPC, SNRPC)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1+ hnRNPC myc was a gift from Gideon Dreyfuss (Addgene plasmid # 71661)