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Addgene

pcDNA3.1+ eiF4A2 myc HIS
(Plasmid #71658)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)/myc-His A
  • Backbone size w/o insert (bp) 5490
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EIF4A2
  • Alt name
    eukaryotic translation initiation factor 4A2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1224
  • GenBank ID
    1974
  • Entrez Gene
    EIF4A2 (a.k.a. BM-010, DDX2B, EIF4A, EIF4F, eIF-4A-II, eIF4A-II)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+ eiF4A2 myc HIS was a gift from Gideon Dreyfuss (Addgene plasmid # 71658 ; http://n2t.net/addgene:71658 ; RRID:Addgene_71658)
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