pcDNA3.1+ eiF4A1 myc HIS
(Plasmid #71657)

• Purpose: mammalian expression of eiF4A1
• Depositing Lab: Gideon Dreyfuss
• Publication: Dreyfuss Lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)/myc-His A
• Backbone size w/o insert (bp): 5490
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eiF4A1
• Alt name: eukaryotic translation initiation factor 4A1
• Species: H. sapiens (human)
• Insert Size (bp): 1221
• Mutation: aa403 from Ala to Arg
• GenBank ID: 1973
• Entrez Gene: EIF4A1 (a.k.a. DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry