pJK657
(Plasmid #71647)

• Purpose: Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)
• Depositing Lab: T. J. Kappock
• Publication: Serine mutants of Acetobacter aceti PurE1 (unpublished)

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4137
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phosphoribosylaminoimidazole carboxylase
• Alt name: purE
• Alt name: TDE0687
• Alt name: aminoimidazole ribonucleotide carboxylase
• Species: Treponema denticola
• Mutation: changes histidine-40 to alanine, phenylalanine-79 to tryptophan
• GenBank ID: NP_971299.1
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The protein produced is anticipated to have no catalytic activity.

This protein is based on the one referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that the active form does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase