pJK511
(Plasmid #71591)

• Purpose: Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
• Depositing Lab: T. J. Kappock
• Publication: Murphy et al Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6410
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: citrate synthase
• Alt name: gltA
• Species: Thermoplasma acidophilum DSM 1728
• Mutation: changes histidine-222 to glutamine
• GenBank ID: TA_RS00855 P21553
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry