pJK438
(Plasmid
#71590)
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PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6410
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecitrate synthase
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Alt namegltA
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SpeciesThermoplasma acidophilum DSM 1728
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GenBank IDTA_RS00855 P21553
- Promoter T7 promoter
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Tag
/ Fusion Protein
- His6 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK438 was a gift from T. J. Kappock (Addgene plasmid # 71590 ; http://n2t.net/addgene:71590 ; RRID:Addgene_71590) -
For your References section:
An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase. Murphy JR, Donini S, Kappock TJ. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23. 10.1107/S2053230X15015939 PubMed 26457521
