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Addgene

pJK594
(Plasmid #71588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4736
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    acetyl-CoA:oxalate CoA-transferase
  • Alt name
    yfdE
  • Entrez Gene
    yfdE (a.k.a. b2371, ECK2367, JW2368)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK594 was a gift from T. J. Kappock (Addgene plasmid # 71588 ; http://n2t.net/addgene:71588 ; RRID:Addgene_71588)

  • For your References section:

    Function and X-ray crystal structure of Escherichia coli YfdE. Mullins EA, Sullivan KL, Kappock TJ. PLoS One. 2013 Jul 23;8(7):e67901. doi: 10.1371/journal.pone.0067901. Print 2013. PONE-D-13-17127 [pii] PubMed 23935849
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