pEGFP-C3_SEPT6
(Plasmid #71583)

• Purpose: mammalian expression of human SEPT6 with a GFP fusion
• Depositing Lab: Elias Spiliotis
• Publication: Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. doi: 10.1016/j.cub.2012.04.019. Epub 2012 May 17.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6008
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Septin 6
• Species: H. sapiens (human)
• Insert Size (bp): 1281
• Mutation: V206D
• Entrez Gene: SEPTIN6 (a.k.a. SEP2, SEPT2, SEPT6)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry