Skip to main content
Addgene

pJK502
(Plasmid #71582)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4115
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphohistidine phosphatase
  • Alt name
    sixA
  • Alt name
    AZ09_02580
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    ACD85599.1 KDE21010.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK502 was a gift from T. J. Kappock (Addgene plasmid # 71582 ; http://n2t.net/addgene:71582 ; RRID:Addgene_71582)

  • For your References section:

    Functional analysis of the acetic acid resistance (aar) gene cluster in Acetobacter aceti strain 1023. Mullins E, Kappock TJ. Acetic Acid Bacteria. 2(s1):e3 (2013) 10.4081/aab.2013.s1.e3
Related items: