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Addgene

pJK564
(Plasmid #71581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71581 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 5108
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    succinyl-CoA:acetate CoA-transferase
  • Alt name
    aarC
  • Alt name
    AZ09_02565
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes serine-71 to alanine
  • GenBank ID
    KDE21007.1 ACD85596.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Much higher protein production is achieved by co-production with E. coli GroEL/GroES.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK564 was a gift from T. J. Kappock (Addgene plasmid # 71581 ; http://n2t.net/addgene:71581 ; RRID:Addgene_71581)

  • For your References section:

    Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases. Mullins EA, Kappock TJ. Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. Epub 2012 Oct 11. 10.1021/bi300957f PubMed 23030530
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