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Addgene

pJK382
(Plasmid #71574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRec7
  • Total vector size (bp) 3764
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    citrate synthase
  • Alt name
    gltA
  • Alt name
    TM
  • Species
    Thermoplasma acidophilum DSM 1728
  • Mutation
    changes aspartate-317 to asparagine; silent mutation in alanine-240 codon.
  • GenBank ID
    TA_RS00855 P21553
  • Promoter recA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site AseI (destroyed during cloning)
  • 5′ sequencing primer lambda-PL promoter
  • 3′ sequencing primer custom
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein production is induced using nalidixic acid as described. (Kurz, L. C., Drysdale, G., Riley, M., Tomar, M. A., Chen, J., Russell, R. J. M., and Danson, M. J. ( 2000) Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum Biochemistry 39, 2283– 2296 DOI: 10.1021/bi991982r)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK382 was a gift from T. J. Kappock (Addgene plasmid # 71574 ; http://n2t.net/addgene:71574 ; RRID:Addgene_71574)

  • For your References section:

    The partial substrate dethiaacetyl-coenzyme A mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by Thermoplasma acidophilum citrate synthase. Kurz LC, Constantine CZ, Jiang H, Kappock TJ. Biochemistry. 2009 Aug 25;48(33):7878-91. doi: 10.1021/bi9006447. 10.1021/bi9006447 PubMed 19645419
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