pJK385
(Plasmid #71572)

• Purpose: Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)
• Depositing Lab: T. J. Kappock
• Publication: Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. doi: 10.1128/JB.00405-08. Epub 2008 May 23.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 5108
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: succinyl-CoA:acetate CoA-transferase
• Alt name: aarC
• Alt name: AZ09_02565
• Species: Acetobacter aceti 1023
• GenBank ID: KDE21007.1 ACD85596.1
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Much higher protein production is achieved by co-production with E. coli GroEL/GroES. See Mullins EA & Kappock TJ (2012) Biochemistry 51:8422-34. doi: 10.1021/bi300957f