pJK277
(Plasmid
#71565)
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PurposeProduces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeast
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYES2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5857
- Total vector size (bp) 6951
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedelta15 fatty acid desaturase
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Alt nameFAD3
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Alt nameFad3
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Alt nameomega-3 fatty acid desaturase
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SpeciesCrepis alpina
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GenBank IDDQ176017 ABA55806
- Promoter GAL1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer CYC1 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK277 was a gift from T. J. Kappock (Addgene plasmid # 71565 ; http://n2t.net/addgene:71565 ; RRID:Addgene_71565) -
For your References section:
Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid. Nam JW, Kappock TJ. J Exp Bot. 2007;58(6):1421-32. Epub 2007 Feb 27. 10.1093/jxb/erm005 PubMed 17329262
