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Addgene

pJK274
(Plasmid #71564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5857
  • Total vector size (bp) 6927
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta12 fatty acid desaturase (form 3)
  • Alt name
    FAD2-3
  • Alt name
    Fad2
  • Species
    Crepis alpina
  • GenBank ID
    DQ289487 ABC00771
  • Promoter GAL1 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer CYC1 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK274 was a gift from T. J. Kappock (Addgene plasmid # 71564 ; http://n2t.net/addgene:71564 ; RRID:Addgene_71564)

  • For your References section:

    Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid. Nam JW, Kappock TJ. J Exp Bot. 2007;58(6):1421-32. Epub 2007 Feb 27. 10.1093/jxb/erm005 PubMed 17329262
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