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Addgene

pJK295
(Plasmid #71561)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71561 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 3954
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    thioredoxin
  • Alt name
    trxA
  • Alt name
    trx
  • Alt name
    AZ09_01835
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE21554 635177282
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK295 was a gift from T. J. Kappock (Addgene plasmid # 71561 ; http://n2t.net/addgene:71561 ; RRID:Addgene_71561)

  • For your References section:

    Atomic-resolution crystal structure of thioredoxin from the acidophilic bacterium Acetobacter aceti. Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ. Protein Sci. 2007 Jan;16(1):92-8. 10.1110/ps.062519707 PubMed 17192591
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