pJK350
(Plasmid #71560)

• Purpose: Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)
• Depositing Lab: T. J. Kappock
• Publication: Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6414
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alanine racemase
• Alt name: alr
• Alt name: AZ09_RS09230
• Species: Acetobacter aceti 1023
• Mutation: mutation changes alanine-365 to aspartate
• GenBank ID: WP_042787947.1 ABC71322.1
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry