Skip to main content
Addgene

pJK345
(Plasmid #71559)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6408
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alanine racemase
  • Alt name
    alr
  • Alt name
    AZ09_RS09230
  • Species
    Acetobacter aceti 1023
  • Mutation
    deletes alanine-367 and alanine-368
  • GenBank ID
    WP_042787947.1 ABC71322.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK345 was a gift from T. J. Kappock (Addgene plasmid # 71559 ; http://n2t.net/addgene:71559 ; RRID:Addgene_71559)

  • For your References section:

    Alanine racemase from the acidophile Acetobacter aceti. Francois JA, Kappock TJ. Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6. 10.1016/j.pep.2006.05.016 PubMed 16843006
Related items: