pEGFP-N1_SEPT2
(Plasmid #71547)

• Purpose: mammalian expression of Sept 2 with a GFP fusion
• Depositing Lab: Elias Spiliotis
• Publication: Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97. doi: 10.1083/jcb.201102076.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5782
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Septin 2
• Species: M. musculus (mouse)
• Insert Size (bp): 1100
• Entrez Gene: Septin2 (a.k.a. AW208991, Nedd-5, Nedd5, Sept2, mKIAA0158)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry