pMpGE011
(Plasmid #71537)

• Purpose: (Empty Backbone) For CRISPR/Cas9 genome editing in Marchantia polymorpha
• Depositing Lab: Takayuki Kohchi
• Publication: Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.

Backbone

• Vector backbone: pMpGWB303
• Backbone manufacturer: Kohchi Lab., Kyoto University
• Backbone size (bp): 13898
• Modifications to backbone: Atco_Cas9 CDS and Pea3A terminator were introduced by LR reaction, an then the attR1-attR2 destination cassette was cloned into the Aor51HI site.
• Vector type: Plant Expression, CRISPR
• Selectable markers: Chlorsulfuron

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • GenBank file
• A portion of this plasmid was derived from a plasmid made by: Holger Puchta at Karlsruhe Institute of Technology, Germany, for the Atco_Cas9 CDS; Tsuyoshi Nakagawa at Shimane University, Japan for the Gateway cassette.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint