pMpGE_En01
(Plasmid
#71534)
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Purpose(Empty Backbone) Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71534 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerThermo
- Backbone size (bp) 2580
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Modifications to backboneA fragment containing MpU6 promoter (2 kb), CmR-ccdB genes flanked by SacI and PstI sites, and gRNA backbone was inserted by TOPO cloning.
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene Plasmid #41824
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMpGE_En01 was a gift from Ikuko Hara-Nishimura & Takayuki Kohchi (Addgene plasmid # 71534 ; http://n2t.net/addgene:71534 ; RRID:Addgene_71534) -
For your References section:
Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. 10.1371/journal.pone.0205117 PubMed 30379827
Map uploaded by the depositor.
