CMV-Cx35-Halo-C-in
(Plasmid #71511)

• Purpose: Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.
• Depositing Lab: John O'Brien
• Publication: Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97. doi: 10.1242/jcs.162586. Epub 2015 Sep 10.

Backbone

• Vector backbone: pcDNA 3.1 zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 7037
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Connexin 35
• Alt name: Cx35
• Alt name: Connexin 36
• Alt name: Cx36
• Species: Morone americana (Perch)
• Insert Size (bp): 2053
• GenBank ID: AF059183.1
• Tag / Fusion Protein:
  • HaloTag (internal to sequence)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry