pSTARR-seq_human
(Plasmid #71509)

• Purpose: (Empty Backbone) humanSTARR-seq screening vector with SCP1 core promoter
• Depositing Lab: Alexander Stark
• Publication: Arnold et al Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. Epub 2013 Jan 17.

Backbone

• Vector backbone: pGL4.10
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression ; humanSTARR-seq screening vector
• Tag / Fusion Protein:
  • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pSTARR-seq_human genbank
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).

The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.