pSTARR-seq_fly-pnr
(Plasmid
#71503)
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Purpose(Empty Backbone) flySTARR-seq screening vector with pnr core promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-Promoter
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Backbone manufacturerPromega
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Vector typeInsect Expression ; flySTARR-seq screening vector
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Tag
/ Fusion Protein
- sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSTARR-seq_fly-pnr was a gift from Alexander Stark (Addgene plasmid # 71503 ; http://n2t.net/addgene:71503 ; RRID:Addgene_71503) -
For your References section:
Enhancer-core-promoter specificity separates developmental and housekeeping gene regulation. Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, Stark A. Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 2014 Dec 15. 10.1038/nature13994 PubMed 25517091