pSTARR-seq_fly-NipB
(Plasmid #71502)

• Purpose: (Empty Backbone) flySTARR-seq screening vector with NipB core promoter
• Depositing Lab: Alexander Stark
• Publication: Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 2014 Dec 15.

Backbone

• Vector backbone: pGL3-Promoter
• Backbone manufacturer: Promega
• Vector type: Insect Expression ; flySTARR-seq screening vector
• Tag / Fusion Protein:
  • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pSTARR-seq_fly-NipB genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.