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Addgene

pRB1083
(Plasmid #71480)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71480 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ601
  • Total vector size (bp) 8113
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VRER Cas9 variant
  • Species
    Synthetic
  • Insert Size (bp)
    4323
  • Promoter eft-3 (eef-1A.1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB1083 was a gift from Andrew Fire (Addgene plasmid # 71480 ; http://n2t.net/addgene:71480 ; RRID:Addgene_71480)

  • For your References section:

    Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Bell RT, Fu BX, Fire AZ. Genetics. 2015 Dec 17. pii: genetics.115.185041. 10.1534/genetics.115.185041 PubMed 26680661
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