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Addgene

pRB1081
(Plasmid #71479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRB1017
  • Total vector size (bp) 2484
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dpy-10 gRNA
  • gRNA/shRNA sequence
    TCCGCTACCATAGGCACCA
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB1081 was a gift from Andrew Fire (Addgene plasmid # 71479 ; http://n2t.net/addgene:71479 ; RRID:Addgene_71479)

  • For your References section:

    Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Bell RT, Fu BX, Fire AZ. Genetics. 2015 Dec 17. pii: genetics.115.185041. 10.1534/genetics.115.185041 PubMed 26680661
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