pMCSG7-H588A-NmeCas9
(Plasmid #71477)

• Purpose: bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site
• Depositing Lab: Erik Sontheimer
• Publication: Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020.

Backbone

• Vector backbone: pMCSG7
• Backbone manufacturer: MCSG
• Backbone size w/o insert (bp): 5286
• Total vector size (bp): 8541
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pMCSG7-H588A-NmeCas9
• Species: Neisseria meningitidis
• Insert Size (bp): 3321
• Mutation: H588A
• Promoter: T7
• Tag / Fusion Protein:
  • 6His (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAC TTC CAA TCC AAT GCC ATG GCT GCC TTC AAA CC
• 3′ sequencing primer: TTA TCC ACT TCC AAT GTT TTA ACG GAC AGG CGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry